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A UV-Vis spectrophotometer is used to determine the absorption of light from a sample and can be used as a detector for HPLC. A sample is placed in the UV/VIS beam, and absorbance versus wavelength is measured. A double beam spectrophotometer utilizes two light beams: a reference beam and a sampling beam that passes through the sample. Some double beam spectrophotometers have two detectors that allow the two beams to be measured simultaneously.
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